Microbiologia Molecular e Microbioma

The Gut-Brain Axis in Alzheimer’s and Parkinson’s Diseases: The Catalytic Role of Mitochondria

Candeias E, 2024. Journal of Alzheimer's Disease. 1 - 17. 2024. http://dx.doi.org/10.3233/jad-240524 . 10.3233/jad-240524 . published Journal of Alzheimer's Disease

Neuronal control of microglia through the mitochondria

Pereira-Santos AR, 2024. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2024. http://dx.doi.org/10.1016/j.bbadis.2024.167167 . 10.1016/j.bbadis.2024.167167 . published Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

Nocardia cyriacigeorgica elicits gut disturbances in a leaky gut model of colitis, but not the harmful cascade leading to gut-first Parkinson’s Disease

Magalhães JD, 2024. International Journal of Molecular Sciences. 2024. https://doi.org/10.3390/ijms25063423 . published International Journal of Molecular Sciences

Gut-brain-axis impact on canine anxiety disorders: new challenges for behavioral veterinary medicine

Sacoor C, 2024. Veterinary Medicine International. 2856759. 2024. 2024. https://doi.org/10.1155/2024/2856759 . published Veterinary Medicine International

Footprints of a microbial toxin from the gut microbiome to mesencephalic mitochondria

Esteves AR; Alarico S, 2023. Gut. 73 - 89. 1. 72. 2023. http://dx.doi.org/10.1136/gutjnl-2021-326023 . 10.1136/gutjnl-2021-326023 . published Gut

Parkinson's Disease: A Multisystem Disorder

Costa HN, 2023. Neuroscience Bulletin. 113 - 124. 1. 39. 2023. http://dx.doi.org/10.1007/s12264-022-00934-6 . 10.1007/s12264-022-00934-6 . published Neuroscience Bulletin

The role of bacteria-mitochondria communication in the activation of neuronal innate immunity: Implications to Parkinson's Disease

Magalhães JD, 2023. International Journal of Molecular Sciences. 5. 24. 2023. http://dx.doi.org/10.3390/ijms24054339 . 10.3390/ijms24054339 . published International Journal of Molecular Sciences

Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides

Maranha A; Pereira PJB, Empadinhas N, 2023. Communications Biology. 1. 6. 2023. https://doi.org/10.1038/s42003-023-04448-3 . 10.1038/s42003-023-04448-3 . published Communications Biology

Sustainable starch-based edible films with agrifood residues as potential carriers for the probiotic Lactobacillus rhamnosus

Coimbra P, 2023. Innovative Food Science & Emerging Technologies. 88. 2023. http://dx.doi.org/10.1016/j.ifset.2023.103452 . 10.1016/j.ifset.2023.103452 . published Innovative Food Science & Emerging Technologies

Effect of ScCO2 on the decontamination of PECs-based cryogels: a comparison with H2O steam and H2O2 nebulization methods

Bento CSA, 2023. International Journal of Pharmaceutics. 2023. http://dx.doi.org/10.1016/j.ijpharm.2023.123451 . 10.1016/j.ijpharm.2023.123451 . published International Journal of Pharmaceutics

Intestinal infection triggers mitochondria-mediated a-synuclein pathology: relevance to Parkinsons disease

Magalhães JD, 2023. Cellular and Molecular Life Sciences. 6. 80. 2023. http://dx.doi.org/10.1007/s00018-023-04819-3 . 10.1007/s00018-023-04819-3 . published Cellular and Molecular Life Sciences

Neurodegenerative Microbially-Shaped Diseases: Oxidative Stress Meets Neuroinflammation

Silva DF, 2022. Antioxidants. 11. 11. 2022. http://dx.doi.org/10.3390/antiox11112141 . 10.3390/antiox11112141 . published Antioxidants

Enzyme promiscuity in serotonin biosynthesis, from bacteria to plants and humans

Gonçalves S, 2022. Frontiers in Microbiology. 13. 2022. http://dx.doi.org/10.3389/fmicb.2022.873555 . 10.3389/fmicb.2022.873555 . published Frontiers in Microbiology

Sequential scCO2 drying and sterilisation of alginate-gelatine aerogels for biomedical applications

Bento C, 2022. The Journal of Supercritical Fluids. 184. 2022. http://dx.doi.org/10.1016/j.supflu.2022.105570 . 10.1016/j.supflu.2022.105570 . published The Journal of Supercritical Fluids

The neuromicrobiology of Parkinson's disease: A unifying theory

Muñoz-Pinto MF, 2021. Ageing Research Reviews. 70. 2021. http://dx.doi.org/10.1016/j.arr.2021.101396 . 10.1016/j.arr.2021.101396 . published Ageing Research Reviews

Improved diabetic wound healing by bovine lactoferricin is associated with relevant changes in the skin immune response and microbiota

Mouritzen MV, 2021. Molecular Therapy - Methods & Clinical Development. 726 - 739. 20. 2021. https://doi.org/10.1016/j.omtm.2021.02.008 . 10.1016/j.omtm.2021.02.008 . published Molecular Therapy - Methods & Clinical Development

The Mitochondrial Ribosome: A World of Opportunities for Mitochondrial Dysfunction Toward Parkinson's Disease

Gonçalves AM, 2021. Antioxidants & Redox Signaling. 694 - 711. 8. 34. 2021. https://www.liebertpub.com/doi/10.1089/ars.2019.7997 . 10.1089/ars.2019.7997 . published Antioxidants & Redox Signaling

Editorial: Interplay Between Nutrition, the Intestinal Microbiota and the Immune System

Oriá RB, 2020. Frontiers in Immunology. 11. 2020. http://dx.doi.org/10.3389/fimmu.2020.01758 . 10.3389/fimmu.2020.01758 . published Frontiers in Immunology

Stabilization of blood for long-term storage can affect antibody-based recognition of cell surface markers

Silva MB; Färnert A, 2020. Journal of Immunological Methods. 481. 2020. http://dx.doi.org/10.1016/j.jim.2020.112792 . 10.1016/j.jim.2020.112792 . published Journal of Immunological Methods

Microbial BMAA elicits mitochondrial dysfunction, innate immunity activation and Alzheimer's disease features in cortical neurons

Silva DF, 2020. Journal of Neuroinflammation. 332. 17. 2020. http://dx.doi.org/10.1186/s12974-020-02004-y . 10.1186/s12974-020-02004-y . published Journal of Neuroinflammation

A genuine mycobacterial thermophile: Mycobacterium hassiacum growth, survival and GpgS stability at near-pasteurization temperatures

Alarico S, 2020. Microbiology. 474 - 483. 5. 166. 2020. http://dx.doi.org/10.1099/mic.0.000898 . 10.1099/mic.0.000898 . published Microbiology

Microbial BMAA and the Pathway for Parkinson's Disease Neurodegeneration

Nunes-Costa D, 2020. Frontiers in Aging Neuroscience. 12. 2020. https://doi.org/10.3389/fnagi.2020.00026 . 10.3389/fnagi.2020.00026 . published Frontiers in Aging Neuroscience

High-Quality Draft Genome Sequences of Rare Nontuberculous Mycobacteria Isolated from Surfaces of a Hospital

Tiago I, 2019. Microbiology Resource Announcements. 21. 8. 2019. https://doi.org/10.1128/MRA.00496-19 . 10.1128/MRA.00496-19 . published Microbiology Resource Announcements

The structural characterization of a glucosylglycerate hydrolase provides insights into the molecular mechanism of mycobacterial recovery from nitrogen starvation

Cereija TB, 2019. IUCrJ. 572 - 585. 4. 6. 2019. https://doi.org/10.1107/S2052252519005372 . 10.1107/S2052252519005372 . published IUCrJ

Biosynthesis of mycobacterial methylmannose polysaccharides requires a unique 1-O-methyltransferase specific for 3-O-methylated mannosides

Ripoll-Rozada J; Pereira PJB; Empadinhas N, 2019. Proceedings of the National Academy of Sciences. 201813450 - 201813450. 2019. https://www.pnas.org/content/116/3/835.long . 10.1073/pnas.1813450116 . published Proceedings of the National Academy of Sciences

Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment

Pereira SG, 2019. BMC Microbiology. 1. 19. 2019. http://dx.doi.org/10.1186/s12866-019-1428-4 . 10.1186/s12866-019-1428-4 . published BMC Microbiology

Molecular Fingerprints for a Novel Enzyme Family in Actinobacteria with Glucosamine Kinase Activity

Manso JA, 2019. mBio. 2019. https://doi.org/10.1128/mBio.00239-19 . 10.1128/mBio.00239-19 . published mBio

The Microbiome-Mitochondria Dance in Prodromal Parkinson's Disease

Cardoso SM, Empadinhas N, 2018. Frontiers in Physiology. 9. 2018. https://www.frontiersin.org/articles/10.3389/fphys.2018.00471/full . 10.3389/fphys.2018.00471 . published Frontiers in Physiology

Production, crystallization and structure determination of a mycobacterial glucosylglycerate hydrolase

Cereija TB, 2017. Acta Crystallographica Section F Structural Biology Communications. 536 - 540. 9. 73. 2017. https://doi.org/10.1107%2Fs2053230x17012419 . 10.1107/S2053230X17012419 . published Acta Crystallographica Section F Structural Biology Communications

Hospital microbial surface colonization revealed during monitoring of Klebsiella spp., Pseudomonas aeruginosa, and non-tuberculous mycobacteria

Farias PG, 2017. Antonie van Leeuwenhoek. 863 - 876. 7. 110. 2017. http://dx.doi.org/10.1007/s10482-017-0857-z . 10.1007/s10482-017-0857-z . published Antonie van Leeuwenhoek

Microbiota of Chronic Diabetic Wounds: Ecology, Impact, and Potential for Innovative Treatment Strategies

Pereira SG, 2017. Frontiers in Microbiology. 8. 2017. https://doi.org/10.3389%2Ffmicb.2017.01791 . 10.3389/fmicb.2017.01791 . published Frontiers in Microbiology

Glucosylglycerate metabolism, bioversatility and mycobacterial survival

Nunes-Costa D, 2016. Glycobiology. 213 - 227. 27. 2016. http://dx.doi.org/10.1093/glycob/cww132 . 10.1093/glycob/cww132 . published Glycobiology

The looming tide of nontuberculous mycobacterial infections in Portugal and Brazil

Nunes-Costa D, 2016. Tuberculosis. 107 - 119. 96. 2016. http://dx.doi.org/10.1016/j.tube.2015.09.006 . 10.1016/j.tube.2015.09.006 . published Tuberculosis

Octanoylation of early intermediates of mycobacterial methylglucose lipopolysaccharides

Maranha A; Clarke AJ, Empadinhas N, 2015. Scientific Reports. 1. 5. 2015. http://dx.doi.org/10.1038/srep13610 . 10.1038/srep13610 . published Scientific Reports

Structure of Mycobacterium thermoresistibile GlgE defines novel conformational states that contribute to the catalytic mechanism

Mendes V, 2015. Scientific Reports. 1. 5. 2015. http://dx.doi.org/10.1038/srep17144 . 10.1038/srep17144 . published Scientific Reports

Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway

Fraga JS, 2015. Scientific Reports. 5. 2015. https://doi.org/10.1038/srep08026 . 10.1038/srep08026 . published Scientific Reports

Autophagy in the fight against tuberculosis

Bento CF, 2014. DNA and Cell Biology. 228 - 242. 4. 34. 2014. https://doi.org/10.1089/dna.2014.2745 . 10.1089/dna.2014.2745 . published DNA and Cell Biology

Mycobacterium hassiacum recovers from nitrogen starvation with up-regulation of a novel glucosylglycerate hydrolase and depletion of the accumulated glucosylglycerate

Alarico S, 2014. Scientific Reports. 1. 4. 2014. http://dx.doi.org/10.1038/srep06766 . 10.1038/srep06766 . published Scientific Reports

The molecular biology of mycobacterial trehalose in the quest for advanced tuberculosis therapies

Nobre A, 2014. Microbiology (United Kingdom). 1547 - 1570. PART 8. 160. 2014. https://doi.org/10.1099/mic.0.075895-0 . 10.1099/mic.0.075895-0 . published Microbiology (United Kingdom)

A new bacterial hydrolase specific for the compatible solutes alpha-D-mannopyranosyl-(1,2)-D-glycerate and alpha-D-glucopyranosyl-(1,2)-D-glycerate

Alarico S, 2013. Enzyme and Microbial Technology. 77 - 83. 2. 52. 2013. http://dx.doi.org/10.1016/j.enzmictec.2012.10.008 . 10.1016/j.enzmictec.2012.10.008 . published Enzyme and Microbial Technology

The plant Selaginella moellendorffii possesses enzymes for synthesis and hydrolysis of the compatible solutes mannosylglycerate and glucosylglycerate

Nobre A, 2013. Planta. 891 - 901. 3. 237. 2013. https://doi.org/10.1007/s00425-012-1808-6 . 10.1007/s00425-012-1808-6 . published Planta

Genome sequence of Mycobacterium hassiacum DSM 44199, a rare source of heat-stable mycobacterial proteins

Tiago I, 2012. Journal of Bacteriology. 7010 - 7011. 24. 194. 2012. DOI: https://doi.org/10.1128/JB.01880-12 . 10.1128/JB.01880-12 . published Journal of Bacteriology

Biosynthesis of mycobacterial methylglucose lipopolysaccharides

Mendes V, 2012. Natural Product Reports. 834 - 844. 8. 29. 2012. https://doi.org/10.1039/C2NP20014G . 10.1039/c2np20014g . published Natural Product Reports

Functional and structural characterization of a novel mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus reveals its dual substrate specificity

Empadinhas N, 2011. Molecular Microbiology. 76 - 93. 1. 79. 2011. https://doi.org/10.1111/j.1365-2958.2010.07432.x . 10.1111/j.1365-2958.2010.07432.x . published Molecular Microbiology

Mycobacterium tuberculosis Rv2419c, the missing glucosyl-3-phosphoglycerate phosphatase for the second step in methylglucose lipopolysaccharide biosynthesis

Mendes V, 2011. Scientific Reports. 1. 1. 2011. http://dx.doi.org/10.1038/srep00177 . 10.1038/srep00177 . published Scientific Reports

Diversity, biological roles and biosynthetic pathways for sugar-glycerate containing compatible solutes in bacteria and archaea

Empadinhas N, da Costa MS, 2011. Environmental Microbiology. 2056 - 2077. 8. 13. 2011. https://doi.org/10.1111/j.1462-2920.2010.02390.x . 10.1111/j.1462-2920.2010.02390.x . published Environmental Microbiology

Two alternative pathways for the synthesis of the rare compatible solute mannosylglucosylglycerate in Petrotoga mobilis

Fernandes C, 2010. Journal of Bacteriology. 1624 - 1633. 6. 192. 2010. https://doi.org/10.1128/JB.01424-09 . 10.1128/JB.01424-09 . published Journal of Bacteriology

Biochemical characterization of the maltokinase from Mycobacterium bovis BCG

Mendes V, 2010. BMC Biochemistry. 2010. https://doi.org/10.1186/1471-2091-11-21 . 10.1186/1471-2091-11-21 . published BMC Biochemistry

Identification of the mycobacterial glucosyl-3-phosphoglycerate synthase

Empadinhas N, 2008. FEMS Microbiology Letters. 195 - 202. 2. 280. 2008. https://doi.org/10.1111/j.1574-6968.2007.01064.x . 10.1111/j.1574-6968.2007.01064.x . published FEMS Microbiology Letters

Crystallization and preliminary crystallographic analysis of mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus

Sá-Moura B, 2008. Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 760 - 763. 8. 64. 2008. https://doi.org/10.1107/S1744309108021490 . 10.1107/S1744309108021490 . published Acta Crystallographica Section F: Structural Biology and Crystallization Communications

A Unique Combination of Genetic Systems for the Synthesis of Trehalose in Rubrobacter xylanophilus: Properties of a Rare Actinobacterial TreT

Nobre A, 2008. Journal of Bacteriology. 7939 - 7946. 24. 190. 2008. http://dx.doi.org/10.1128/jb.01055-08 . 10.1128/jb.01055-08 . published Journal of Bacteriology

Osmoadaptation mechanisms in prokaryotes: Distribution of compatible solutes

Empadinhas N, da Costa MS, 2008. International Microbiology. 151 - 161. 3. 11. 2008. https://europepmc.org/article/med/18843593 . 10.2436/20.1501.01.55 . published International Microbiology

Mycobacterium tuberculosis glucosyl-3-phosphoglycerate synthase: Structure of a key enzyme in methylglucose lipopolysaccharide biosynthesis

Pereira PJB, 2008. PLoS ONE. 11. 3. 2008. https://doi.org/10.1371/journal.pone.0003748 . 10.1371/journal.pone.0003748 . published PLoS ONE

Molecular and physiological role of the trehalose-hydrolyzing a-glucosidase from Thermus thermophilus HB27

Alarico S, 2008. Journal of Bacteriology. 2298 - 2305. 7. 190. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-41549086172&partnerID=MN8TOARS . 10.1128/JB.01794-07 . published Journal of Bacteriology

To be or not to be a compatible solute: Bioversatility of mannosylglycerate and glucosylglycerate

Empadinhas N, da Costa MS, 2008. Systematic and Applied Microbiology. 159 - 168. 3. 31. 2008. https://doi.org/10.1016/j.syapm.2008.05.002 . 10.1016/j.syapm.2008.05.002 . published Systematic and Applied Microbiology

Single-step pathway for synthesis of glucosylglycerate in Persephonella marina

Fernandes C, 2007. Journal of Bacteriology. 4014 - 4019. 11. 189. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249786874&partnerID=MN8TOARS . 10.1128/JB.00075-07 . published Journal of Bacteriology

Organic solutes in Rubrobacter xylanophilus: The first example of di-myo-inositol-phosphate in a thermophile

Empadinhas N, 2007. Extremophiles. 667 - 673. 5. 11. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548303944&partnerID=MN8TOARS . 10.1007/s00792-007-0084-z . published Extremophiles

Mannosylglycerate is essential for osmotic adjustment in Thermus thermophilus strains HB27 and RQ-1

Alarico S, 2007. Extremophiles. 833 - 840. 6. 11. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-35748948644&partnerID=MN8TOARS . 10.1007/s00792-007-0106-x . published Extremophiles

Glucosylglycerate biosynthesis in the deepest lineage of the Bacteria: Characterization of the thermophilic proteins GpgS and GpgP from Persephonella marina

Costa J, 2007. Journal of Bacteriology. 1648 - 1654. 5. 189. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947366922&partnerID=MN8TOARS . 10.1128/JB.00841-06 . published Journal of Bacteriology

Bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol: Inositol-1-phosphate transferase, the key enzyme for di-myo-inositol-phosphate synthesis in several (hyper)thermophiles

Rodrigues MV, 2007. Journal of Bacteriology. 5405 - 5412. 15. 189. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547629796&partnerID=MN8TOARS . 10.1128/JB.00465-07 . published Journal of Bacteriology

Characterization of the biosynthetic pathway of glucosylglycerate in the archaeon Methanococcoides burtonii

Costa J, 2006. Journal of Bacteriology. 1022 - 1030. 3. 188. 2006. http://www.scopus.com/inward/record.url?eid=2-s2.0-31344453995&partnerID=MN8TOARS . 10.1128/JB.188.3.1022-1030.2006 . published Journal of Bacteriology

Diversity and biosynthesis of compatible solutes in hyper/thermophiles

Empadinhas N, da Costa MS, 2006. International Microbiology. 199 - 206. 3. 9. 2006. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750584278&partnerID=MN8TOARS . published International Microbiology

Meiothermus timidus sp. nov., a new slightly thermophilic yellow-pigmented species

Pires AL, 2005. FEMS Microbiology Letters. 39 - 45. 1. 245. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-15744405109&partnerID=MN8TOARS . 10.1016/j.femsle.2005.02.011 . published FEMS Microbiology Letters

Distribution of genes for synthesis of trehalose and mannosylglycerate in Thermus spp. and direct correlation of these genes with halotolerance

Alarico S, 2005. Applied and Environmental Microbiology. 2460 - 2466. 5. 71. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-18444382346&partnerID=MN8TOARS . 10.1128/AEM.71.5.2460-2466.2005 . published Applied and Environmental Microbiology

A gene from the mesophilic bacterium Dehalococcoides ethenogenes encodes a novel mannosylglycerate synthase

Empadinhas N, 2004. Journal of Bacteriology. 4075 - 4084. 13. 186. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-3042523198&partnerID=MN8TOARS . 10.1128/JB.186.13.4075-4084.2004 . published Journal of Bacteriology

Specialized Roles of the Two Pathways for the Synthesis of Mannosylglycerate in Osmoadaptation and Thermoadaptation of Rhodothermus marinus

Borges N, 2004. Journal of Biological Chemistry. 9892 - 9898. 11. 279. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-1642279326&partnerID=MN8TOARS . 10.1074/jbc.M312186200 . published Journal of Biological Chemistry

The Bacterium Thermus thermophilus , Like Hyperthermophilic Archaea, Uses a Two-Step Pathway for the Synthesis of Mannosylglycerate

Empadinhas N, 2003. Applied and Environmental Microbiology. 3272 - 3279. 6. 69. 2003. http://dx.doi.org/10.1128/aem.69.6.3272-3279.2003 . 10.1128/aem.69.6.3272-3279.2003 . published Applied and Environmental Microbiology

Gamma-Proteobacteria Aquicella lusitana gen. nov., sp. nov., and Aquicella siphonis sp. nov. Infect Protozoa and Require Activated Charcoal for Growth in Laboratory Media

Santos P, 2003. Applied and Environmental Microbiology. 6533 - 6540. 11. 69. 2003. http://www.scopus.com/inward/record.url?eid=2-s2.0-0242573382&partnerID=MN8TOARS . 10.1128/AEM.69.11.6533-6540.2003 . published Applied and Environmental Microbiology

Rubritepida flocculans gen. nov., sp. nov., a New Slightly Thermophilic Member of the a-1 Subclass of the Proteobacteria

Alarico S, 2002. Systematic and Applied Microbiology. 198 - 206. 2. 25. 2002. http://dx.doi.org/10.1078/0723-2020-00116 . 10.1078/0723-2020-00116 . published Systematic and Applied Microbiology

Albidovulum inexpectatum gen. nov., sp. nov., a nonphotosynthetic and slightly thermophilic bacterium from a marine hot spring that is very closely related to members of the photosynthetic genus Rhodovulum

Albuquerque L, 2002. Applied and Environmental Microbiology. 4266 - 4273. 9. 68. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036729238&partnerID=MN8TOARS . 10.1128/AEM.68.9.4266-4273.2002 . published Applied and Environmental Microbiology

Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii: Biochemical and genetic characterization of key enzymes

Empadinhas N, 2001. Journal of Biological Chemistry. 43580 - 43588. 47. 276. 2001. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035941355&partnerID=MN8TOARS . 10.1074/jbc.M108054200 . published Journal of Biological Chemistry

Biosynthesis of mannosylglycerate in the thermophilic bacterium Rhodothermus marinus. Biochemical and genetic characterization of a mannosylglycerate synthase

Martins LO, 1999. Journal of Biological Chemistry. 35407 - 35414. 50. 274. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033544948&partnerID=MN8TOARS . 10.1074/jbc.274.50.35407 . published Journal of Biological Chemistry