The Mass Spectrometry Laboratory provides advanced mass spectrometry approaches to resolve multiple life sciences related questions, ranging from targeted small molecule quantification to intact protein analysis, and with a major focus on large scale quantitative proteomics. The facility is specialized in the identification and quantification of proteins from complex samples, identification of post-translational modifications, and identification and quantification of metabolites. It is mainly focused on running R&D projects with partners and collaborators (from CiBB, Biocant, other R&D institutes and private companies). The team is also actively involved in training, through Masters and PhD courses at the University of Coimbra, namely with the Chemistry Department, the Life Sciences Department, the Faculty of Medicine and CNC-UC, among others.
The Mass Spectrometry Laboratory is part of the RNEM- Portuguese Mass Spectrometry Network that integrates the RNIE, and a member of the European Research Infrastructure INSTRUCT.
Keywords
Quantitative proteomics; Small molecule quantification; Drug-metabolites identification and quantification; Post translational modifications; Intact protein analysis
Services / Technologies
Proteomics Analysis
• Protein/Peptide Identification (Protein Pilot)
• Protein/Peptide Relative quantification by SWATH-MS (PeakView – SWATH quantitation tool)
• Post-translational modifications (Protein Pilot)
• Intact protein analysis
• Protein/Peptide quantification by MRM
Small molecules Analysis
• Untargeted analysis (Full scan and/or SWATH-MS)
Small molecules identification and relative quantification (MarkerView, MasterView) - Drug metabolites identification (Metabolite Pilot)
• Targeted analysis
Absolute quantification of compounds (drugs, aminoacids, lipids, etc.) by MRM (Multiquant)
Statistical Analysis
• Univariate and Multivariate analysis can be provided
List of Available Equipment
Triple TOF 5600 with an Eksigent nanoUltra LC
Triple TOF 6600 with an Eksigent 425 LC
4000 QTRAP with a Shimadzu Nexera
Certification
ISO 9001
