The Flow Cytometry Unit provides a range of services including sample analysis and cell sorting, as well as equipment, software and technical expertise/consultancy for a variety of flow cytometry protocols. Its aim is to enhance the quality and scope of scientific research by providing high quality, cost-effective, state-of-the-art flow cytometry and multi-parameter cell sorting to all CiBB researchers and, by appointment, to those outside CiBB. The facility, with two sites, UC-Polo I in Coimbra and UC-Biotech in Cantanhede, offers state-of-the-art cell sorters capable of sorting up to four different cell populations simultaneously, and flow cytometer analysers capable of performing multi-parameter flow cytometric analysis.
Keywords
Flow cytometry; Cell sorting; Cell analysis
Services / Technologies
Cell sorting;
Data acquisition, analysis and interpretation;
User training;
Advice on sample preparation;
User assistance and machine troubleshooting;
Help with design of experiments;
Sourcing of reagents and techniques and finding alternative approaches;
Help with data preparation for presentation and publication;
We are also open to collaboration.
List of Available Equipment
Becton Dickinson FACSCalibur cell analyser (Polo I);
Partec CyFlow® Space cell sorter (Polo I);
Becton Dickinson Accuri™ C6 cell analyser (UC-Biotech) with auto-sampler;
Becton Dickinson FACSAria III cell sorter (UC-Biotech).
Contacts
flow-poloI@cnc.uc.pt (Polo I) or flow@uc-biotech.pt (UC-Biotech)
