Intracellular trafficking
Neuronal disorders & Ageing
Drosophila
Mitochondria
Live microscopy
Regulation of intracellular trafficking during neuronal ageing and neurodegeneration
Membrane contact sites organisation during neuronal ageing and neurodegeneration
CRISPR/Cas9 genetic screens in Drosophila to study developmental and ageing trajectories
The work of my research group explores the complexity of neuronal biology, focusing on understanding how intracellular trafficking influences neuronal function, and its role in healthy aging and neurodegeneration.
We combine in vivo studies in Drosophila melanogaster with cultured mammalian neurons, coupled to advanced microscopy approaches, behavioural assays, and tool development. We also wish to interrogate the fly genome to find potential new determinants of organismal fitness. We hope our work will offer novel insights into avenues of intervention for age-related and neurodegenerative conditions by contributing significantly to our understanding of neuronal biology.
For more information, please visit the lab website www.vagnonilab.com
Information about journal articles, updated at 12-04-2026, from platform CIÊNCIAVITAE.
A SPLICS reporter reveals alpha-synuclein regulation of lysosome-mitochondria contacts which affects TFEB nuclear translocation
Giamogante, Flavia; Barazzuol, Lucia; Maiorca, Francesca; Poggio, Elena; Esposito, Alessandra; Masato, Anna; Napolitano, Gennaro; et al, 2024. Nature Communications. 1. 15. 2024. http://dx.doi.org/10.1038/s41467-024-46007-2 . 10.1038/s41467-024-46007-2 . Nature Communications
Yeast NDI1 reconfigures neuronal metabolism and prevents the unfolded protein response in mitochondrial complex I deficiency
Lucy Granat; Daniel Ranson; Debbra Knorr; Emma Hamer; Ram Prosad Chakrabarty; Francesca Mattedi; Laura Fort-Aznar; et al, 2023. PLoS genetics. 7. 19. 2023. https://kclpure.kcl.ac.uk/portal/en/publications/e56f87c3-f1df-4c21-9777-93ff9290ee08 . https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010793 . PLoS genetics
Live Imaging of Mitochondria in the Intact Fly Wing
Francesca Mattedi; Daniel C Maddison; Gaynor Ann Smith; Alessio Vagnoni, 2023. Cold Spring Harbor protocols. 106 - 112. 2. 2023. 2023. https://kclpure.kcl.ac.uk/portal/en/publications/395cb895-70d8-4030-ae2d-2f0b6ffe1f36 . 10.1101/pdb.prot108052 . Cold Spring Harbor protocols
Optogenetic cleavage of the Miro GTPase reveals the direct consequences of real-time loss of function in Drosophila.
Mattedi F; Lloyd-Morris E; Hirth F; Alessio Vagnoni, 2023. PLoS biology. 2023. https://europepmc.org/articles/PMC10465005 . 10.1371/journal.pbio.3002273 . PLoS biology
Detailed imaging of mitochondrial transport and precise manipulation of mitochondrial function with genetically-encoded photosensitizers in adult Drosophila neurons
Alessio Vagnoni; Francesca Mattedi; George Chennell, 2022. Methods in Molecular Biology. 2022. https://kclpure.kcl.ac.uk/portal/en/publications/db7b9b0a-5a10-4850-ac42-b4581948757d . https://doi.org/10.1007/978-1-0716-1990-2_20 . Methods in Molecular Biology
Clonal Imaging of Mitochondria in the Dissected Fly Wing
Daniel C Maddison; Francesca Mattedi; Alessio Vagnoni; Gaynor Ann Smith, 2022. Cold Spring Harbor protocols. 10.1101/pdb.prot1080 - 10.1101/pdb.prot1080. 2022. https://kclpure.kcl.ac.uk/portal/en/publications/157d758a-dbfd-4627-a162-af43d8d565d3 . 10.1101/pdb.prot108051 . Cold Spring Harbor protocols
Decreasing pdzd8-mediated mito-ER contacts improves organismal fitness and mitigates Aß42 toxicity
Victoria L. Hewitt; Leonor Miller-Fleming; Madeleine J. Twyning; Simonetta Andreazza; Francesca Mattedi; Julien Prudent; Franck Polleux; Alessio Vagnoni; Alexander J. Whitworth, 2022. Life Science Alliance. 11. 5. 2022. https://kclpure.kcl.ac.uk/portal/en/publications/061bca72-7653-4ca8-ab50-a29ca8a89ebd . 10.26508/lsa.202201531 . Life Science Alliance
High-resolution imaging of mitochondria and mitochondrial nucleoids in differentiated SH-SY5Y cells
Alessio Vagnoni; Emily Annuario; Kristal Ng, 2022. Methods in Molecular Biology. 2022. https://kclpure.kcl.ac.uk/portal/en/publications/ca005c0e-a0f6-4d8f-b8f2-7285adf6311e . https://doi.org/10.1007/978-1-0716-1990-2_15 . Methods in Molecular Biology
Analysis of Mitochondrial Dynamics in Adult
Daniel C Maddison; Francesca Mattedi; Alessio Vagnoni; Gaynor Ann Smith, 2022. Cold Spring Harbor protocols. 2022. https://kclpure.kcl.ac.uk/portal/en/publications/d26c7c3c-0dbf-4381-839b-385325735fdb . 10.1101/pdb.top107819 . Cold Spring Harbor protocols
LMTK2 binds to kinesin light chains to mediate anterograde axonal transport of cdk5/p35 and LMTK2 levels are reduced in Alzheimer's disease brains
Mórotz, G.M.; Glennon, E.B.; Gomez-Suaga, P.; Lau, D.H.W.; Robinson, E.D.; Sedlák, É.; Vagnoni, A.; Noble, W.; Miller, C.C.J., 2019. Acta neuropathologica communications. 73 - 73. 1. 7. 2019. http://www.scopus.com/inward/record.url?eid=2-s2.0-85065767031&partnerID=MN8TOARS . 10.1186/s40478-019-0715-5 . Acta neuropathologica communications
Temporal Control of Axonal Transport: The Extreme Case of Organismal Ageing
Mattedi, F.; Vagnoni, A., 2019. Frontiers in Cellular Neuroscience. 13. 2019. http://www.scopus.com/inward/record.url?eid=2-s2.0-85072726742&partnerID=MN8TOARS . 10.3389/fncel.2019.00393 . Frontiers in Cellular Neuroscience
Kinesin light chain-1 serine-460 phosphorylation is altered in Alzheimer's disease and regulates axonal transport and processing of the amyloid precursor protein
Gábor M. Mórotz; Elizabeth Glennon; Jenny Greig; Dawn H.W. Lau; Nishita Bhembre; Francesca Mattedi; Nadine Muschalik; et al, 2019. Acta Neuropathologica Communications. 1. 7. 2019. https://kclpure.kcl.ac.uk/portal/en/publications/0c203dbd-a636-4d1d-8e1c-44d785dd0c1a . 10.1186/s40478-019-0857-5 . Acta Neuropathologica Communications
A cAMP/PKA/Kinesin-1 Axis Promotes the Axonal Transport of Mitochondria in Aging Drosophila Neurons
Alessio Vagnoni; Simon L. Bullock, 2018. Current Biology. 2018. https://kclpure.kcl.ac.uk/portal/en/publications/8b022c26-5c06-4a9e-813a-3e0fd14bc5a3 . 10.1016/j.cub.2018.02.048 . Current Biology
Methodological advances in imaging intravital axonal transport [version 1; referees: 3 approved]
James N. Sleigh; Alessio Vagnoni; Alison E. Twelvetrees; Giampietro Schiavo, 2017. 2017. https://f1000research.com/articles/6-200/v1 . 10.12688/f1000research.10433.1 .
Reducing Lissencephaly-1 levels augments mitochondrial transport and has a protective effect in adult Drosophila neurons
Vagnoni, A.; Hoffmann, P.C.; Bullock, S.L., 2016. Journal of Cell Science. 178 - 190. 1. 129. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84959017047&partnerID=MN8TOARS . 10.1242/jcs.179184 . Journal of Cell Science
A simple method for imaging axonal transport in aging neurons using the adult Drosophila wing
Vagnoni, A.; Bullock, S.L., 2016. Nature Protocols. 1711 - 1723. 9. 11. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-85015187726&partnerID=MN8TOARS . 10.1038/nprot.2016.112 . Nature Protocols
Loss of c-Jun N-terminal kinase-interacting protein-1 does not affect axonal transport of the amyloid precursor protein or Aß production
Vagnoni, A.; Glennon, E.B.C.; Perkinton, M.S.; Gray, E.H.; Noble, W.; Miller, C.C.J., 2013. Human Molecular Genetics. 4646 - 4652. 22. 22. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84887005627&partnerID=MN8TOARS . 10.1093/hmg/ddt313 . Human Molecular Genetics
Lemur tyrosine kinase-2 signalling regulates kinesin-1 light chain-2 phosphorylation and binding of Smad2 cargo
Manser, C.; Guillot, F.; Vagnoni, A.; Davies, J.; Lau, K.-F.; McLoughlin, D.M.; De Vos, K.J.; Miller, C.C.J., 2012. Oncogene. 2773 - 2782. 22. 31. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84859607330&partnerID=MN8TOARS . 10.1038/onc.2011.437 . Oncogene
Amyotrophic lateral sclerosis-associated mutant VAPBP56s perturbs calcium homeostasis to disrupt axonal transport of mitochondria
Mórotz, G.M.; De Vos, K.J.; Vagnoni, A.; Ackerley, S.; Shaw, C.E.; Miller, C.C.J., 2012. Human Molecular Genetics. 1979 - 1988. 9. 21. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84859246580&partnerID=MN8TOARS . 10.1093/hmg/dds011 . Human Molecular Genetics
Cdk5/p35 phosphorylates lemur tyrosine kinase-2 to regulate protein phosphatase-1C phosphorylation and activity
Manser, C.; Vagnoni, A.; Guillot, F.; Davies, J.; Miller, C.C.J., 2012. Journal of Neurochemistry. 343 - 348. 3. 121. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84859597153&partnerID=MN8TOARS . 10.1111/j.1471-4159.2012.07650.x . Journal of Neurochemistry
Calsyntenin-1 mediates axonal transport of the amyloid precursor protein and regulates aß production
Vagnoni, A.; Perkinton, M.S.; Gray, E.H.; Francis, P.T.; Noble, W.; Miller, C.C.J., 2012. Human Molecular Genetics. 2845 - 2854. 13. 21. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863518335&partnerID=MN8TOARS . 10.1093/hmg/dds109 . Human Molecular Genetics
Phosphorylation of kinesin light chain 1 at serine 460 modulates binding and trafficking of calsyntenin-1
Vagnoni, A.; Rodriguez, L.; Manser, C.; De Vos, K.J.; Miller, C.C., 2011. Journal of Cell Science. 1032 - 1042. 7. 124. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79953141458&partnerID=MN8TOARS . 10.1242/jcs.075168 . Journal of Cell Science
Riluzole protects against glutamate-induced slowing of neurofilament axonal transport
Stevenson, A.; Yates, D.M.; Manser, C.; De Vos, K.J.; Vagnoni, A.; Leigh, P.N.; McLoughlin, D.M.; Miller, C.C.J., 2009. Neuroscience Letters. 161 - 164. 2. 454. 2009. http://www.scopus.com/inward/record.url?eid=2-s2.0-62949103631&partnerID=MN8TOARS . 10.1016/j.neulet.2009.02.061 . Neuroscience Letters
5 23 out of 23 Publications
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